Flavobacterium erciyesense sp. nov., a putative non-pathogenic fish symbiont


Satıcıoğlu İ. B.

ARCHIVES OF MICROBIOLOGY, vol.203, no.9, pp.5783-5792, 2021 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 203 Issue: 9
  • Publication Date: 2021
  • Doi Number: 10.1007/s00203-021-02566-2
  • Journal Name: ARCHIVES OF MICROBIOLOGY
  • Journal Indexes: Science Citation Index Expanded, Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, Environment Index, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.5783-5792
  • Keywords: Antimicrobial resistance genes, Aquaculture, Flavobacterium, Oncorhynchus mykiss, Rainbow trout, EMENDED DESCRIPTION, GENOME, IDENTIFICATION, FAMILY

Abstract

During a study aiming at isolation and genomic characterization of non-pathogenic fish symbionts, strain F-328(T) was isolated from skin mucus of healthy-seeming rainbow trout (Oncorhynchus mykiss) and subjected to polyphasic characterization, including a comprehensive genome analysis. Strain F-328(T) was Gram stain negative, none-gliding, strictly aerobic, and rod shaped. The 16S rRNA gene sequence of strain F-328(T) exhibited the highest level of identity (98.9%) with F. turcicum F-339(T). The major fatty acids were iso-C-15:0, C-15:1 omega 6c, and summed feature 3 (C-16:1 omega 7c, C-16:1 omega 6c). The polar lipid profile consisted mainly of phosphatidylethanolamine and aminolipids, while the predominant menaquinone was MK-6. The DNA G + C content and genome size of the strain were 35.3% and 3.4 Mb, respectively. Strain F-328(T) shows 93.8% average nucleotide identity and 53% digital DNA-DNA hybridization identity with the closest type strain F. turcicum F-339(T). In addition, strain F-328(T) was found to carry antimicrobial resistance genes, which confer resistance to several antimicrobials, including aminoglycoside, macrolides, and streptogramin. Differential phenotypic properties, together with the phylogenetic inference, demonstrate that strain F-328(T) should be classified as a novel species of the genus Flavobacterium for which the name Flavobacterium erciyesense sp. nov. is proposed, with F-328(T) as the type strain (= JCM 34201(T) = KCTC 82261(T)).