Genotoxicity testing of the herbicide trifluralin and its commercial formulation treflan using the piscine micronucleus test

Koenen S., Cavas T.

ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, vol.49, no.6, pp.434-438, 2008 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 49 Issue: 6
  • Publication Date: 2008
  • Doi Number: 10.1002/em.20401
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.434-438
  • Keywords: trifluralin, treflan, genotoxicity, micronucleus test, Oreochromis niloticus, IN-VIVO GENOTOXICITY, DNA-DAMAGE, PERIPHERAL ERYTHROCYTES, FISH CELLS, ASSAY, INGREDIENTS, GLYPHOSATE, PESTICIDES, POLLUTION, ROUNDUP
  • Bursa Uludag University Affiliated: No


In this the genotoxic effects of a widely used herbicide, trifluralin, and its commercial formulation, Treflan, were evaluated using the micronucleus test in a commercially important fish species, Oreochromis niloticus (Nile Tilapia). Fish were exposed to 1, 5, and 10 mu g/L doses of trifluralin and Treflan for 3, 6, and 9 days under laboratory conditions. Ethylmethanesulfonate, at a single dose of 10 mg/L, was used as positive control. Micronuclei were evaluated on the peripheral erythrocytes. Both Treflan and trifluralin treatments significantly increased the micronucleus frequencies in peripheral erythrocytes of O. niloticus. Furthermore, the genotoxicity of the active ingredient, trifluralin, was observed to be higher than that of the commercial formulation Treflan. Our results indicate that herbicide trifluralin has genotoxic potential in fish.