Optimization of Extraction of Rutin From Butcher's Broom Fruit by Central Composite Design

Aybastıer Ö.

Anatolian Congress 6th International Applied Sciences Congress, Van, Turkey, 21 - 23 May 2021, pp.68

  • Publication Type: Conference Paper / Summary Text
  • City: Van
  • Country: Turkey
  • Page Numbers: pp.68
  • Bursa Uludag University Affiliated: Yes


Ruscus aculeatus, commonly called butcher's broom, piaranthus or knee holly, is a mounded, prickly, evergreen sub-shrub that is native to the Black Sea area, Europe and northern Africa. It is widely planted in gardens, and has spread as a garden escapee in many areas outside its native range. Butcher's broom has been used to treatment for a variety of diseases. It was also used to diuretic and reduce swelling and to speed the recovery of fractures since ancient times. Recent research has also uncovered that there are phenolic compounds present in butcher's broom may be physiologically active as an antioxidant. Butcher's broom fruit exhibits antioxidant potential connected to its flavonoid content. Flavonoids have also been shown to possess antioxidant, antiviral, antimicrobial, anticarcinogenic properties. Because phenolic compounds and flavonoids differ in chemical structure and matrix complexity, extraction conditions must be optimized for each. Many factors contribute to the extraction efficiency, such as the temperature solvent type and concentration, time, pH and particle size.
In this study, ultrasonic-assisted extraction of rutin was optimized using central composite design (CCD). The CCD was employed to evaluate the effects of temperature, extraction time, methanol concentration and HCl concentration on the extraction of rutin. The optimum extraction conditions were determined by quantitative determination of rutin in the extract. The amounts of rutin in the extract after extraction were determined by HPLC-DAD. The optimum conditions were determined as temperature 69 oC, extraction time 119 min, methanol concentration 47% (v/v) and HCl concentration 0.30 M. It has been observed that the rutin is in the maximum amount under optimum conditions. The developed method can be applied to plants for the extraction of rutin.