COMBINED EFFECT OF GEMCITABIN AND DUBERMATINIB (TP-0903) ON APOPTOTIC MARKERS IN PANC‑1 CELL LINE

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Kara H., Atıcı Y., Altunok T. H., Sarıoğlu Bozkurt A., Sönmez Ö., Bayram E., ...More

35. Ulusal Biyokimya Kongresi, Antalya, Turkey, 28 October - 01 November 2024, vol.49, no.1, pp.171, (Summary Text)

  • Publication Type: Conference Paper / Summary Text
  • Volume: 49
  • City: Antalya
  • Country: Turkey
  • Page Numbers: pp.171
  • Bursa Uludag University Affiliated: Yes

Abstract

COMBINED EFFECT OF GEMCITABIN AND DUBERMATINIB (TP-0903) ON APOPTOTIC MARKERS IN PANC‑1 CELL LINE

 

Hatibe KARA1, Yasemin ATICI2, Tuğba Hazal ALTUNOK1, Aybike SARIOĞLU BOZKURT1, Öner SÖNMEZ1, Elif BAYRAM1, Ahsenur SEVİM1

1 Bursa Uludağ Üniversitesi Veteriner Fakültesi Biyokimya Anabilim Dalı, Bursa, Türkiye

2 Lokman Hekim Üniversitesi Tıp Fakültesi Tıbbi Biyokimya Anabilim Dalı, Ankara, Türkiye

Objectives: 

Pancreatic cancer is considered as one of the aggressive malignancies due to poor prognosis and resistance to standard chemotherapeutic agents such as gemcitabine (GEM). TP-0903 is one of the Axl inhibitors that is a member of the Receptor Tyrosine Kinases family. In this study, the combined effect of GEM and TP-0903 on apoptotic markers in Panc-1 cell line was investigated.

Materials and Methods: Panc-1 cells were treated with GEM, TP-0903 and their combination. SRB test was performed to determine the cytotoxic effect. Quantitative analysis of expression levels of apoptotic markers Bcl-2 and Caspase-3 were determined by TaqMan RT-PCR method.

Results: In the Panc-1 cell line, the cytotoxic effects of 0.5µM GEM and 20nM TP-0903, when applied individually, were compared with their combined application. After 72 hours of incubation, the combined treatment exhibited a higher cytotoxic effect compared to GEM and TP-0903 applied separately. However, no significant changes were observed in Bcl-2 and Caspase-3 expression levels after 24, 48, and 72 hours of incubation with GEM, TP-0903, or the GEM+TP combination.

Conclusions: These findings suggest that Panc-1 cells may possess resistance to cell death through apoptotic pathways under certain conditions. Investigations into alternative cell death pathways may provide further insights into the underlying mechanism of the observed cytotoxic effect.

Acknowledgements: This study was supported by Bursa Uludağ University Scientific Research Projects Coordination Office with project number TGA-2024-1461. We would like to thank them for their support to the Project.

Keywords: Panc-1, gemcitabine, dubermatinib, apoptosis, RT-PCR