In this study, we examined the influence of hydrogen peroxide, wounding and IBA on the rooting success of chestnut hardwood cuttings. Cuttings of European chestnut genotypes 51111 (Sariaslama Clone -1) and 51205 (Sariaslama Clone -2), were treated with IBA at 0, 12000 ppm and H2O2 (3.5% w/v) solutions. Cuttings were prepared 14-16 cm in length in January, wounding the opposite sides of the base of the cutting. Compressed peat pellet was used as rooting media. Presence of callus and rooting rate were determined.