The relationship between CRP gene polymorphism (rs2794521, rs3091244), ASDAS-CRP and ASDAS-ESR in ankylosing spondylitis


Aksoy M. K. , Altan L., Gorukmez O., Guner A., Ayar K.

MODERN RHEUMATOLOGY, vol.30, no.4, pp.715-720, 2020 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 30 Issue: 4
  • Publication Date: 2020
  • Doi Number: 10.1080/14397595.2019.1639916
  • Journal Name: MODERN RHEUMATOLOGY
  • Journal Indexes: Science Citation Index Expanded, Scopus, Academic Search Premier, EMBASE, MEDLINE
  • Page Numbers: pp.715-720
  • Keywords: Ankylosing spondylitis, CRP gene polymorphism, ASDAS CRP, ASDAS ESR, acute phase reactants, C-REACTIVE PROTEIN, DISEASE-ACTIVITY, ASSOCIATION, HLA-B27, SPONDYLOARTHRITIS, PREDICTION, PROMOTER, CANCER, ALLELE, SNP

Abstract

Background: We aimed to investigate the haplotypes and alleles of two variants (rs2794521 and rs3091244) in AS patients and to examine their relationship with ASDAS-CRP and ASDAS-ESR values. Methods: We evaluated 160 AS patients diagnosed according to the ASAS criteria. ASDAS-CRP and ASDAS-ESR values were calculated. ESR and CRP were examined. The restriction fragment length polymorphism (RFLP) method was used for detecting the rs2794521 and rs3091244 regions on the CRP gene. Results: As a result of the evaluation of rs2794521 gene polymorphism using PCR, TT, TC and CC genotypes were observed in 90, 81 and 9 individuals, respectively. As a result of the evaluation of rs3091244 gene polymorphism, CC, AC and TT genotypes were observed in 104, 51 and 5 individuals, respectively. T allele and C allele were found in rs2794521 gene by 75% and 25%, respectively. In addition, T allele, C allele and A allele were found in rs3091244 gene by 80%, 17% and 3%, respectively. With the help of regression equation, ASDAS-CRP level was 0.34 units higher in cases with rs3091244 C allele than cases without rs3091244 C alleles. Conclusion: CRP rs3091244 C allele may be associated with the increased relative risk for ASDAS-CRP.