Karadut tohumlarının çimlenmesi üzerine prolin ve sıcaklık uygulamalarının etkileri


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Kaçal E., Çalışkan O., Atak A., Aydınlı M., Öztürk G., Bayav A.

MUSTAFA KEMAL UNIVERSITY JOURNAL OF AGRICULTURAL SCIENCES (MKUJAS), vol.25, no.2, pp.181-188, 2020 (Peer-Reviewed Journal)

Abstract

Aims: Black mulberry (Morus nigra) is a species that difficult to reproduce as vegetative and generative. For this reason, different applications are made by researchers in order to germinate their seeds more easily. In this study, we aimed to determine the effects of proline applications on germination of black mulberry seeds under various temperature conditions. Methods and Results: The seeds were kept in room temperature for 24 hours, in 1 mM, 5 mM, 10 mM and 15 mM proline solutions and in the control group in the water. At the end of this period, they were placed in sterile petri dishes with three replicates of 25 seeds. Seed germination studies were carried out in plant growth cabins at 15°C, 20°C, 25°C and 30°C temperatures in dark conditions. At the end of the 17-day germination period, the germination rate of the seeds in the control group varied between 28% and 70.67%, the highest germination rate was achieved at 20oC. In proline, the highest germination rate was determined with 93.33% in 10 mM proline and seeds kept at 25oC. In general, the germination rate reached the highest value on days 13 and 14 in all applications except 15oC. Conclusions: In this study, it has been determined that black mulberry seeds germinate high rate at the 20oC without any application. This rate has exceeded 90% with the 10 mM proline at 25oC. Significance and Impact of the Study: The use of black mulberry seedlings in the production of saplings and obtaining hybrid plants in the recent hybridization breeding studies depend on the success of seed reproduction. In this study, 10 mM proline application at 25oC was found to be very successful in reducing seed loss and increasing the germination rate.