Akademik Veri Yönetim Sistemi
IV. BALKAN AGRICULTURAL CONGRESS - AGRIBALKAN 2022 , Edirne, Türkiye, 31 Ağustos - 02 Eylül 2022, ss.156
Tomatoes leaf miner (Tuta absoluta) is one of the most important constraints to tomato production. The use of entomopathogen fungi would be a cost-effective solution to control any pest. However, the pathogenicity and virulence difference among entomopathogenic fungi, Beauvaria and Metarhizium isolates and concentrations are not widely well investigated. The objectives of this study were therefore initiated to test the pathogenicity of some entomopathogenic fungus isolates against Tuta absoluta. The experiments were conducted at Bursa Uludag University, Turkey and Kulumsa Agricultural research center, Ethiopia laboratory commencing from 2021 to 2022. Tuta absoluta adults were collected and were reared on tomato seedling in growth chamber. The third instar larvae of Tuta absolute were inoculated with four Ethiopia EPF isolates (B1, PPRC-56, M1 and M2) and four EPF Turkish isolates (AK-10, AK-14 AK-11 and AK-12) at different conidia concentrations (0, 106, 107,108,109 and 1010 conidia/ml ) in a factorial experiment arranged in completely randomized design with three replications. Mortality rate assessment data was conducted and analyzed. The analysis of variance for mortality percentage revealed significant variations (p<0.05) among EPF species isolates and conidia concentrations. The results revealed that Beauvaria bassiana isolates were found to show the highest mortality rate 70% to 81%, the lowest LC50, 1.87x104 to 9.79x104 conidia/ml, LC95, 5.68x108 to 1.10x1011 conidia/ml and shortest incubation period LT50 ,3.6 to 5.7 days , highly virulent against Tuta absolute and should be preferred to be used. Metarhizium anisopliae include M1, M2, AK-11 and AK-12 were determined as moderately virulent against Tuta absoluta having mortality rate 46 to 63 %, the highest LC50, 1.36x106 to 4.94x107 and LT50, 4.3 to 8 However, among all isolates “Metarhizium anisopliae AK-12 ” showed the least mortality 46 %, has the highest LC50 value, 4.94x107 followed by “Metarhizium anisopliae AK-11. Hence, the effectiveness of Beauvaria bassania isolates were more than Metarhizium anisopliae . Results also revealed that among EPF concentrations 1x1010 is the most effective dose rate while 1x106 is the least effective to kill larvae. As the current work revealed the potential variation among EPF species isolates and conidia concentrations effectively against Tuta absolute, further isolates that showed moderately virulent should be further evaluated against Tuta absoluta.