EXPERIMENTAL STUDIES ON INVITRO MATURATION OF PORCINE OOCYTES .2. EFFECTS OF ESTRADIOL-17-BETA AND PROGESTERONE


EROGLU A.

BERLINER UND MUNCHENER TIERARZTLICHE WOCHENSCHRIFT, vol.106, no.5, pp.157-159, 1993 (Journal Indexed in SCI) identifier

  • Publication Type: Article / Article
  • Volume: 106 Issue: 5
  • Publication Date: 1993
  • Title of Journal : BERLINER UND MUNCHENER TIERARZTLICHE WOCHENSCHRIFT
  • Page Numbers: pp.157-159

Abstract

The simple compounded semi-defined Whitten's medium was supplemented with estradiol-17beta and progesterone to examine whether they influence the nuclear maturation of porcine cumulus-oocyte complexes. Addition of estradiol-17beta at concentrations of 1.0 mug/ml and 10.0 mug/ml significantly inhibited the nuclear maturation in comparison to the control group. No significant inhibition of estradiol-17beta was observed at concentration of 0.1 mug/ml with respect to the control. After addition of synchronous or subsequent (12 h after the incubation with estradiol-17beta) supplementation of progesterone at concentration of 1 mug/ml, maturation rates were as high as control. So progesterone overcame the inhibitory effect of estradiol-17beta.