Salmonella Enteritidis predominance determined by serotyping and real-time PCR in poultry-derived food and avian isolates


Ata Z., TEMELLİ S. , Eyigor A. G. , CARLI K. T.

TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, vol.41, no.2, pp.187-192, 2017 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 41 Issue: 2
  • Publication Date: 2017
  • Doi Number: 10.3906/vet-1604-35
  • Title of Journal : TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
  • Page Numbers: pp.187-192

Abstract

This study aimed to determine Salmonella enterica subspecies enterica serovar Enteritidis (SE) presence by conventional serotyping and SE-specific real-time PCR (SE-rPCR) in poultry-derived food and avian isolates in our laboratory Salmonella spp. collection. Conventional serotyping indicated that 32 (8 chicken meat, 10 egg, 14 avian) out of 56 (57%) isolates were SE, whereas 8 (3 chicken meat, 2 turkey meat, 3 avian) (14%) isolates were serogroup B, 6 (1 chicken meat, 1 egg, 4 avian) (11%) were serogroup C1, 4 (3 chicken meat, 1 turkey meat) (7%) were serogroup C2-C3, 4 (3 chicken meat, 1 turkey meat) (7%) were serogroup E4, and 2 (avian) (14%) isolates were categorized as nonserogrouped/nonserotyped. Thirty-three (8/18 chicken meat, 10/11 egg, 15/23 avian) out of 56 (59%) Salmonella isolates were positive by SE-rPCR. SE was determined as the most prevalent serotype in both of the tests regardless of the sample type. Conventional serotyping and SE-rPCR results were in agreement in all but 1 isolate. Considerably high relative accuracy (98%), sensitivity (100%), and specificity (96%) with almost perfect agreement between the two methods (Cohen's kappa = 0.96) indicated SE-rPCR as a reliable tool in the rapid identification of SE isolates to complement conventional serotyping.