Microscopic and molecular detection of Nosema spp. in honeybees of Turkey

ÜTÜK A. E., Piskin F. C., GİRİŞGİN A. O., Selcuk O., AYDIN L.

APIDOLOGIE, cilt.47, sa.2, ss.267-271, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 47 Sayı: 2
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1007/s13592-015-0394-6
  • Dergi Adı: APIDOLOGIE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.267-271
  • Anahtar Kelimeler: Nosema apis, Nosema ceranae, Multiplex PCR, Turkey, APIS-MELLIFERA, MICROSPORIDIAN PARASITE, CERANAE, BEE, INFECTION, EUROPE
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

In this study, we aimed to determine the prevalence of Nosema spp. in honeybees of Turkey. For this aim, adult honeybee (Apis mellifera) samples were collected from 1621 colonies within 95 apiaries located in 22 provinces of Turkey. Samples were examined microscopically. In case of positivity, spore identification was done by multiplex PCR. At the end of microscopic examination, Nosema spp. spores were detected in 7 out of 22 provinces (31.8 %), and 16 out of 95 colonies (16.8 %) that represent 1621 colonies. According to PCR results, 1 out of 16 isolates (6.25 %) was Nosema apis, and 15 out of 16 isolates (93.75 %) were Nosema ceranae. The result of our study indicated that N.ceranae is the dominant species in Turkey.