Effects of the bovine SLICK1 mutation in PRLR on sweat gland area, FOXA1 abundance, and global gene expression in skin


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Sosa F., Carmickle A., Oliveira L., Sagheer M., Saleem M., Yu F., ...More

Journal of Dairy Science, vol.105, no.11, pp.9206-9215, 2022 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 105 Issue: 11
  • Publication Date: 2022
  • Doi Number: 10.3168/jds.2022-22272
  • Journal Name: Journal of Dairy Science
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Periodicals Index Online, Agricultural & Environmental Science Database, Analytical Abstracts, BIOSIS, Business Source Elite, Business Source Premier, CAB Abstracts, Chemical Abstracts Core, EMBASE, Environment Index, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database, Directory of Open Access Journals, DIALNET
  • Page Numbers: pp.9206-9215
  • Keywords: prolactin, prolactin receptor, skin, SLICK1 mutation
  • Bursa Uludag University Affiliated: Yes

Abstract

© 2022 American Dairy Science AssociationThe SLICK1 mutation in the prolactin receptor (PRLR) results in a short-hair coat and increased ability to regulate body temperature during heat stress. It is unclear whether the mutation affects capacity for sweating. The objective of this observational study was to evaluate whether the SLICK1 mutation in PRLR alters characteristics of skin related to sweat gland abundance or function. Skin biopsies from 31 Holstein heifers, including 14 wild-type (SL−/−) and 17 heterozygous slick (SL+/−), were subjected to histological analysis to determine the percent of the surface area of skin sections that are occupied by sweat glands. We detected no effect of genotype on this variable. Immunohistochemical analysis of the forkhead transcription factor A1 (FOXA1), a protein essential for sweating in mice, from 6 SL−/− and 6 SL+/− heifers indicated twice as much FOXA1 in sweat glandular epithelia of SL+/− heifers as in SL−/− heifers. Results from RNA sequencing of skin biopsies from 5 SL−/− and 7 SL+/− heifers revealed few genes that were differentially expressed and none that have been associated with sweat gland development or function. In conclusion, results do not support the idea that the SLICK1 mutation changes the abundance of sweat glands in skin, but do show that functional properties of sweat glands, as indicated by increased abundance of immunoreactive FOXA1, are modified by inheritance of the mutation in PRLR.