Effects Of Pyrimidines On Viability, Proliferation And Polarization In RAW 264.7 Macrophage Cell Line


Ermiş E., Etgü O., Özalp E., Oral H. B., Yöyen Ermiş D., Cansev M.

5th International Molecular Immunology & Immunogenetics Congress, İzmir, Türkiye, 20 - 22 Ekim 2022, ss.133

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: İzmir
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.133
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

Introduction: Macrophages are the first line of defense against pathogens and their dysfunction (such as M1/M2phenotype and proliferation) can lead to serious health problems. Macrophage proliferation capasity, can be adversely affected by diseases such as cancer, infection or autoimmune diseases. Pyrimidines such as UMP,UDP,UTP are endogenous organic compounds.Previous studies showed that exogenously administered UMP,UDP,UTP stimulate the movement of monocytes and macrophages to the disease area and enhance phagocytic activities of macrophages.It was also previously demonstrated that pyrimidines enhance the growth, differentiation and proliferation of nerve cells in vitro.However, effects of UMP, UDP, UTP on the proliferation and polarization of macrophages have not yet been investigated. Method: Raw264.7 mouse cell lines were transferred into tissue culture treated 6-well-plates and grown to 100%confluence. Upon reaching confluence, the cells in monolayer were vertically scratched using a tip. Uridine, UMP,UDP,UTP diluted in culture media was added to individual wells relevant doses ranging from 1,10,100μM. Images were captured every 24hours over a 3days period using an inverted light microscope to observe cellular migration(wound closure) and analysed by ImajeJ software. Proliferation effect of the Uridine,UMP,UDP,UTP on Raw 264.7 was determined using MTT assay. Cell viability was calculated as percentage change in comparison to the OD values obtained from untreated control cells.CD11b and CD206 expression were tested by flow cytomety.

Conclusion: According to MTT and scratch assay results, especially uridine and UTP were found to support proliferation at the end of 72 hours incubation. The percentage of CD11b+CD206+ cells were also increased in response to UTP and uridine stimulation.