Development of in-vitro produced bovine embryos in four different culture media.


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Doğan İ., Sağırkaya H. , Soylu M. K. , Nur Z.

Indian Journal Of Animal Research, vol.72, no.3, pp.227-229, 2002 (Journal Indexed in SCI Expanded)

  • Publication Type: Article / Article
  • Volume: 72 Issue: 3
  • Publication Date: 2002
  • Title of Journal : Indian Journal Of Animal Research
  • Page Numbers: pp.227-229

Abstract

The objective of this study was to compare the de\eIopmentai ability of embryos in 4 different media, CR I aa,

modified CR2, BCEM-g and TCM-199. Bovine oocytes were matured and then fertilized in vitro. A total of915 oocytes

were used for fertilizatiqn, 681 (74%) of which/cleaved, The 2-cell embryos were divided into 4 groups, and cultured in

I of the 4 different culture media. Percentage of development to morula-blastocyst stage from the 2 cell stage were

determined as 46. 31,30 and 28% for CRI aa, CR2, BCEM-g and TCM-I99 culture media respectively. The development

rate obtained with CR I aa was significantly'higher (P<O.O I) compared to that with the other media.