RAPD analysis of DNA polymorphism in turkish sheep breeds

Elmaci C., Oner Y., Ozis Ş., Tuncel E.

BIOCHEMICAL GENETICS, cilt.45, sa.9-10, ss.691-696, 2007 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 45 Sayı: 9-10
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1007/s10528-007-9106-x
  • Dergi Adı: BIOCHEMICAL GENETICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.691-696
  • Anahtar Kelimeler: sheep, DNA, polymorphism, RAPD, PCR, ARBITRARY PRIMERS, MARKERS, POPULATIONS, GENOME, GENE, PCR
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

The genetic diversity and phylogenetic relationships of 108 individual sheep from three Turkish sheep breeds (Kivircik, Gokceada, and Sakiz) were studied using RAPD analysis. Polymorphisms within and between populations were assayed using 15 random primers, and 82 loci were amplified ranging from 250 to 2,500 bp. The percentage of polymorphic loci was found to be 80.49, 78.05, and 73.17%, for Kivircik, Gokceada, and Sakiz sheep breeds, respectively. Total genetic diversity was 0.2265, and the average coefficient of genetic differentiation was 0.1181. Genetically, the Gokceada breed was more closely related to the Sakiz breed than to the Kivircik breed.