RAPD analysis of DNA polymorphism in turkish sheep breeds


Elmaci C. , Oner Y. , Ozis Ş. , Tuncel E.

BIOCHEMICAL GENETICS, vol.45, no.9-10, pp.691-696, 2007 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 45 Issue: 9-10
  • Publication Date: 2007
  • Doi Number: 10.1007/s10528-007-9106-x
  • Title of Journal : BIOCHEMICAL GENETICS
  • Page Numbers: pp.691-696
  • Keywords: sheep, DNA, polymorphism, RAPD, PCR, ARBITRARY PRIMERS, MARKERS, POPULATIONS, GENOME, GENE, PCR

Abstract

The genetic diversity and phylogenetic relationships of 108 individual sheep from three Turkish sheep breeds (Kivircik, Gokceada, and Sakiz) were studied using RAPD analysis. Polymorphisms within and between populations were assayed using 15 random primers, and 82 loci were amplified ranging from 250 to 2,500 bp. The percentage of polymorphic loci was found to be 80.49, 78.05, and 73.17%, for Kivircik, Gokceada, and Sakiz sheep breeds, respectively. Total genetic diversity was 0.2265, and the average coefficient of genetic differentiation was 0.1181. Genetically, the Gokceada breed was more closely related to the Sakiz breed than to the Kivircik breed.