Comparison of Mycoplasma gallisepticum Infection in Different Samples and Ages of Chicken Breeder Flocks


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Demirbilek S. , Ardicli O., Carli K. T.

BRAZILIAN JOURNAL OF POULTRY SCIENCE, vol.22, no.2, 2020 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 22 Issue: 2
  • Publication Date: 2020
  • Doi Number: 10.1590/1806-9061-2020-1271
  • Title of Journal : BRAZILIAN JOURNAL OF POULTRY SCIENCE
  • Keywords: Culture, egg yolk, Mycoplasma gallisepticum, real-time PCR, tracheal swab, EGG TRANSMISSION, DIAGNOSIS, SYNOVIAE, PCRS

Abstract

This study aimed to compare method-based and newly developed sample-based methods for Mycoplasma gallisepticum (MG) detection in different samples of breeder flocks suffering from respiratory disease problems by using culture, real-time PCR (rPCR) and ELISA from chicks and embryonated eggs. Overall, 450 samples of 19-day-old chicken embryo's trachea, 450 samples of 8-day-old chicken tracheal swabs and 900 blood samples of 20-, 27-, 34-, 40- and 46-week-old breeder chickens from 5 flocks were sampled for 26 weeks, and were all tested for MG by culture, MG-rPCR and MG-ELISA. Culturing assays and rPCR were applied to 450 mixture samples from 19-day-old chicken embryo's trachea and 450 tracheal swab samples (each pooled into groups of 3) from 8-day-old chicks from the same flocks. Also, 900 blood samples from the same 5 breeder flocks suffering from respiratory disease problems were tested by MG-ELISA.