Acaricidal, repellent and oviposition deterrent activities of Datura stramonium L. against adult Tetranychus urticae (Koch)


JOURNAL OF PEST SCIENCE, vol.83, no.2, pp.173-180, 2010 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 83 Issue: 2
  • Publication Date: 2010
  • Doi Number: 10.1007/s10340-009-0284-7
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.173-180
  • Keywords: Acaricidal, Oviposition deterrent, Repellent, Spider mite, Thorn apple, 2-SPOTTED SPIDER-MITE, AZADIRACHTIN, RESISTANCE
  • Bursa Uludag University Affiliated: Yes


The ethanol extracts obtained from both leaf and seed in the Thorn apple (Datura stramonium L.) (Solanaceae) were investigated for acaricidal, repellent and oviposition deterrent properties against adult two-spotted spider mites (Tetranychus urticae Koch) (Acari: Tetranychidae) under laboratory conditions. Leaf and seed extracts, which were applied in 167,250 and 145,750 mg/l concentrations, respectively (using a Petri leaf disc-spray tower method), caused 98 and 25% mortality among spider mite adults after 48 h. The simple logistic regression analysis showed that the independent variable, an increase in the dose of leaf extract was associated with a significant increase in the death rate of T. urticae females, but an increase in the dosage of seed extracts did not have a significant effect. Using probit analysis and estimating the parameters with a confidence limit of 95%, we determined the LC(50) values of leaf extract to be 70,593 mg/l. According to Pearson's chi(2) test, mites showed the strongest run off to bean leaf surfaces sprayed with both leaf and seed extracts (in sub-lethal doses: < 7,500 mg/l and < 25,000 mg/l, respectively) and moved towards surfaces that had not been sprayed with extracts. Furthermore, repeated measures ANOVA indicated a significant difference between the number of eggs laid on unsprayed bean leaves compared to bean leaves that were sprayed with leaf and seed extracts at sub-lethal doses, 2,500 and 25,000 mg/l concentrations, respectively. These results suggest that D. stramonium extracts could be used to manage the two-spotted spider mite.