Cyclone separators have been extensively used both for particle sampling and particle removal from fluids, air or process gases in the field of air pollution control and in industry. In this experimental study, effects of inlet velocity, inlet shape and cyclone length on the cyclone collection efficiency and pressure losses were investigated. A new cyclone design without conical part and its performance were also presented. Experiments show that optimum inlet size and cyclone length can be found for better collection efficiency at a specified flow rate. Experimental results from the tests on the new cyclone are evaluated and compared with the results in conventional cyclone. Results show that the new cyclone gives better efficiency comparing to the conventional cyclone for the same cyclone diameter and inlet velocity.