Research Information System
European Journal of Integrative Medicine, vol.75, 2025 (SCI-Expanded)
Introduction: Lichens are symbiotic associations that live in diverse environmental conditions. They produce metabolites such as carotenoids, lichen phenolics, and water-soluble phenolics. The study aims to determine the content and in-vitro biological activities of Peltigera lichens from Bursa province, which have not been previously investigated. Methods: Methanol and acetone extracts were analyzed. Liquid Chromatography-Time of Flight Mass Spectrometry (LC/QTOF/MS) was used for qualitative and quantitative analysis. In-vitro antioxidant and tyrosinase inhibitory activities were evaluated, along with cytotoxic activity assessments. Results: Zeaxanthin was identified in the acetone extract of P. neckeri. Vanillic acid was found to be the major component in both P. canina extracts, while neoxanthin was detected in the methanol extract. Evernic acid and usnic acid were also present in both extracts. Rosmarinic acid, rutin, and zeaxanthin were not detected in P. ponojensis extracts, whereas neoxanthin, usnic acid, and evernic acid were present. The most active extract was the methanol extract of P. ponojensis, with an IC50 value of 23.52 ± 0.02 µg/mL for radical scavenging. No tyrosinase inhibitory activity was observed in either extract of P. neckeri. The most active extract was methanol extract of P. ponojensis with an IC50 value of 30.588 ± 0.02 µg/mL. Cytotoxicity results showed no death or proliferation reduction in any tested cell lines. Conclusion: Peltigera species may serve as potential sources of antioxidants.