Lactoferrin and oxidized low-density lipoprotein receptor 1 genotypes in relation to fattening performance and carcass traits in Simmental bulls


Ardıçlı S. , Şamlı H. , Dinçel D. , Karalar B., Balcı F.

Uluslararası Tıp ve Sağlık Bilimleri Kongresi'ne (INCOMES), İstanbul, Turkey, 26 - 28 April 2021, vol.1, no.114, pp.248-249

  • Publication Type: Conference Paper / Summary Text
  • Volume: 1
  • City: İstanbul
  • Country: Turkey
  • Page Numbers: pp.248-249

Abstract

This study aimed to estimate the effects of lactoferrin (LTF) and oxidized low-density lipoprotein receptor 1 (OLR1) genes on fattening performance and carcass traits. The analysis included a total of 74 Simmental bulls that were raised in a private farm in the South Marmara region of Turkey. Genomic DNA extraction was performed using the phenol-chloroform method. A PCR-RFLP method using EcoRI and PstI enzyme digestion was employed to identify the genotypes in LTF and OLR1, respectively. The frequencies of genotypes and alleles were determined, and thus, population genetic evaluation was performed including Hardy-Weinberg equilibrium, gene heterozygosity, the number of effective alleles, polymorphism information content, fixation index. The phenotypic traits analyzed were the final weight, fattening period, total weight gain, average daily weight, hot carcass weight, chilled carcass weight, dressing percentage, and chilling loss. The association analysis was carried out using the least square methods of the general linear model procedure. Results revealed that both loci showed deviations from Hardy–Weinberg expectation (P<0.05). In LTF, there were only three BB animals and hence this genotype was excluded from the association analyses. Concerning the OLR1 gene, the vast majority of animals had the heterozygous genotype. Population genetic parameters indicated that the LTF EcoRI and the OLR1 PstI markers are moderately informative markers in Simmentals. The association between OLR1 PstI polymorphism and dressing percentage was found to be statistically significant (P<0.05). The AA animals had a higher dressing percentage compared to the CC and heterozygous genotype carriers. There was no significant association between the LTF EcoRI polymorphism and any of the phenotypic traits analyzed in this study. Although verification in further studies is needed, the present analysis may be useful in the evaluation of the effective markers on the fattening performance of cattle.