The extracts obtained from six Lactarius species [Lactarius deterrimus Grager, Lactarius sanguifluus (Paul.: Fr.) Fr., Lactarius semisanguifluus Heim et Leclair, Lactarius piperatus Scop. ex Fr., Lactarius deliciosus (L. ex Fr.) S. F. Gray and Lactarius salmonicolor Heim et Leclair] have been investigated for their antimicrobial activity. Growth inhibition using agar disk diffusion assays was determined against: Escherichia coli ATCC 11230, Micrococcus luteus ATCC 2971, Stapylococcus aureus ATCC 6538P, Salmonella thyphi ATCC 19430, Klebsiella pneumoniae UC57, Pseudomonas aeruginosa ATCC 27853, Corynebacterium xerosis CCM 2824, Bacillus cereus ATCC 7064, Bacillus megaterium DSM 32, Mycobacterium smegmatis CCM 2067, Candida albicans ATCC10231 and Saccharomyces cerevisiae ATCC 9763. As a result of this study, we have found that Lactarius species revealed antimicrobial activity against some Gram (+) and Gram (-) bacteria, but showed no antagonistic effect against yeasts used in this study.