Effect of ß-glucan from Euglena gracilis as an antioxidant on goat semen cryopreservation


ÜSTÜNER B., YILMAZ M. E., Huraydin O., Cimen T., SONAT F., Aktar A., ...Daha Fazla

Journal of the Hellenic Veterinary Medical Society, cilt.75, sa.2, ss.7361-7370, 2024 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 75 Sayı: 2
  • Basım Tarihi: 2024
  • Doi Numarası: 10.12681/jhvms.34504
  • Dergi Adı: Journal of the Hellenic Veterinary Medical Society
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.7361-7370
  • Anahtar Kelimeler: antioxidant activity, DNA fragmentation, Goat, mitochondrial membrane potential, ß-glucan
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

This study aimed to determine the influence of different doses of β-glucan on post-thaw spermatological parameters, lipid peroxidation, and total antioxidant activity of buck semen. In the non-breeding season, semen was collected from bucks twice weekly. After then, ejaculates were pooled and divided into four equal aliquots: β-glucan concentrations of 1 mM (βG1), 2 mM (βG2), and 4 mM (βG4), and a control group without antioxidants. Each sample group was diluted for cryopreservation using a dilution method involving two steps. The experimental groups were then evaluated for several parameters including sperm motility, plasma membrane functional integrity [hypoosmotic swelling test (HOST)], damaged acrosome rate [FITC-Pisum sativum agglutinin (FITC-PSA)], DNA integrity [terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)], mitochondrial membrane potential using JC-1, evaluation of lipid peroxidation (Malondialdehyde; MDA) and determination of total antioxidant capacity; TAC). The post-thaw motility and plasma functional integrity of the control group were significantly lower than those values in the βG groups (P < 0.05). Although the numerically greatest acrosome damage was detected in the control group, it was only statistically different from βG1 and βG4 (P<0.05). While the DNA fragmentation rate of the control group was higher than βG4 group (P<0.05), it was similar to βG1 and βG2 groups (P>0.05). There was no statistical difference among all the groups regarding low mitochondrial membrane potential, MDA, and TAC rates. In line with our results, supplementation of 1mM, 2 mM and 4 mM β-glucan to freezing extender improves the post-thaw spermatological characteristics of goat semen.