Effect of ß-glucan from Euglena gracilis as an antioxidant on goat semen cryopreservation
JOURNAL OF THE HELLENIC VETERINARY MEDICAL SOCIETY, cilt.75, sa.2, ss.7361-7370, 2024 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 75 Sayı: 2
- Basım Tarihi: 2024
- Doi Numarası: 10.12681/jhvms.34504
- Dergi Adı: JOURNAL OF THE HELLENIC VETERINARY MEDICAL SOCIETY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, Veterinary Science Database
- Sayfa Sayıları: ss.7361-7370
- Anahtar Kelimeler: antioxidant activity, DNA fragmentation, Goat, mitochondrial membrane potential, ß-glucan
- Bursa Uludağ Üniversitesi Adresli: Evet
Özet
This study aimed to determine the influence of different doses of B-glucan on post-thaw spermatological parameters, lipid peroxidation, and total antioxidant activity of buck semen. In the non-breeding season, semen was collected from bucks twice weekly. After then, ejaculates were pooled and divided into four equal aliquots: B-glucan concentrations of 1 mM (BG1), 2 mM (BG2), and 4 mM (BG4), and a control group without antioxidants. Each sample group was diluted for cryopreservation using a dilution method involving two steps. The experimental groups were then evaluated for several parameters including sperm motility, plasma membrane functional integrity [hypoosmotic swelling test (HOST)], damaged acrosome rate [FITC-Pisum sativum agglutinin (FITC-PSA)], DNA integrity [terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)], mitochondrial membrane potential using JC-1, evaluation of lipid peroxidation (Malondialdehyde; MDA) and determination of total antioxidant capacity; TAC). The post-thaw motility and plasma functional integrity of the control group were significantly lower than those values in the BG groups (P < 0.05). Although the numerically greatest acrosome damage was detected in the control group, it was only statistically different from BG1 and BG4 (P<0.05). While the DNA fragmentation rate of the control group was higher than BG4 group (P<0.05), it was similar to BG1 and BG2 groups (P>0.05). There was no statistical difference among all the groups regarding low mitochondrial membrane potential, MDA, and TAC rates. In line with our results, supplementation of 1mM, 2 mM and 4 mM B-glucan to freezing extender improves the post-thaw spermatological characteristics of goat semen.