The asphaltene has been obtained from asphalt cement with penetration grade 60, which is extracted from crude Libya petroleum. The elemental composition and some structural properties of the asphaltene samples are determined by various methods, such as SEM, infrared spectroscopy, and X-ray analysis. Some experiments are performed on 1H dynamic nuclear polarization (DNP) in a number of asphaltene suspensions in organic solvents. The frequency dependence of the DNP enhancement under conditions of weak EPR saturation in a low magnetic field is obtained and interpreted as the EPR line shape of some free radicals present in the samples. Copyright © Taylor & Francis Group, LLC.