Positive effect of autologous platelet rich plasma on Saanen buck semen cryopreservation in non-breeding season


ALÇAY S. , Aktar A., KOCA D. , KILIÇ M., Akkasoglu M., SAĞIRKAYA H.

Cryobiology, vol.103, pp.45-48, 2021 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 103
  • Publication Date: 2021
  • Doi Number: 10.1016/j.cryobiol.2021.09.012
  • Title of Journal : Cryobiology
  • Page Numbers: pp.45-48
  • Keywords: Platelet-rich plasma, Buck semen, Cryopreservation, Post-thaw, EGG-YOLK, SOYBEAN LECITHIN, INCUBATION RESILIENCE, OXIDATIVE STRESS, POSTTHAW QUALITY, SPERM, ANTIOXIDANTS, EXTENDERS, FROZEN, FERTILITY

Abstract

© 2021 Elsevier Inc.The objective of the current study was to evaluate the effect of autologous platelet-rich plasma (PRP) addition into soybean lecithin based extender on buck semen at post-thaw. Semen samples were collected from eight Saanen buck, and each semen sample was split into four equal aliquots and diluted with different concentrations of PRP supplemented extenders [no PRP (control), 0.5 × 107/ml PRP, 1 × 107/ml PRP, or 2 × 107/ml PRP]. Motility, plasma membrane functional integrity, acrosome integrity, mitochondrial membrane potential, DNA integrity and malondialdehyde concentrations (MDA) were measured and analyzed at post-thaw. The results showed that 2 × 107/ml PRP group had a positive effect on motility (62.41 ± 4.24), membrane functional integrity (71.11 ± 2.90), mitochondrial membrane potential (69.70 ± 1.99), DNA integrity (7.22 ± 0.93) and MDA levels (2.56 ± 0.73) at post-thaw (P < 0.05). The results of the study demonstrated that autologous PRP has a protective effect on cryopreservation of buck spermatozoa and the fertility effects are worthy of further study.