Positive effect of autologous platelet rich plasma on Saanen buck semen cryopreservation in non-breeding season

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Atıf İçin Kopyala

ALÇAY S., Aktar A., KOCA D., KILIÇ M., Akkasoglu M., SAĞIRKAYA H.

Cryobiology, cilt.103, ss.45-48, 2021 (SCI-Expanded)

• Yayın Türü: Makale / Tam Makale
• Cilt numarası: 103
• Basım Tarihi: 2021
• Doi Numarası: 10.1016/j.cryobiol.2021.09.012
• Dergi Adı: Cryobiology
• Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Artic & Antarctic Regions, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database
• Sayfa Sayıları: ss.45-48
• Anahtar Kelimeler: Platelet-rich plasma, Buck semen, Cryopreservation, Post-thaw, EGG-YOLK, SOYBEAN LECITHIN, INCUBATION RESILIENCE, OXIDATIVE STRESS, POSTTHAW QUALITY, SPERM, ANTIOXIDANTS, EXTENDERS, FROZEN, FERTILITY
• Bursa Uludağ Üniversitesi Adresli: Evet

Özet

© 2021 Elsevier Inc.The objective of the current study was to evaluate the effect of autologous platelet-rich plasma (PRP) addition into soybean lecithin based extender on buck semen at post-thaw. Semen samples were collected from eight Saanen buck, and each semen sample was split into four equal aliquots and diluted with different concentrations of PRP supplemented extenders [no PRP (control), 0.5 × 107/ml PRP, 1 × 107/ml PRP, or 2 × 107/ml PRP]. Motility, plasma membrane functional integrity, acrosome integrity, mitochondrial membrane potential, DNA integrity and malondialdehyde concentrations (MDA) were measured and analyzed at post-thaw. The results showed that 2 × 107/ml PRP group had a positive effect on motility (62.41 ± 4.24), membrane functional integrity (71.11 ± 2.90), mitochondrial membrane potential (69.70 ± 1.99), DNA integrity (7.22 ± 0.93) and MDA levels (2.56 ± 0.73) at post-thaw (P < 0.05). The results of the study demonstrated that autologous PRP has a protective effect on cryopreservation of buck spermatozoa and the fertility effects are worthy of further study.