Higher nisin yield is reached with glutathione and pyruvate compared with heme in Lactococcus lactis N8

Ersoy Z., Kayihan C., Tunca S.

BRAZILIAN JOURNAL OF MICROBIOLOGY, cilt.51, sa.3, ss.1247-1257, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 51 Sayı: 3
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1007/s42770-019-00216-w
  • Dergi Adı: BRAZILIAN JOURNAL OF MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database, Directory of Open Access Journals
  • Sayfa Sayıları: ss.1247-1257
  • Anahtar Kelimeler: Glutathione, Lactococcus lactis, Nisin production, Oxidative stress, Pyruvate, RESPIRATION CAPACITY, HYDROGEN-PEROXIDE, OXIDATIVE-STRESS, GENE-EXPRESSION, NADH OXIDASE, ACID, IDENTIFICATION, GROWTH, RESISTANCE, REDUCTASE
  • Bursa Uludağ Üniversitesi Adresli: Hayır

Özet

There are different studies that aim to enhance the production of nisin by Lactococcus lactis since its chemical synthesis is not possible. In this study, glutathione (GSH) and pyruvate, which are known to reduce the oxidative stress of cells, have been shown to trigger the production of nisin at both transcriptional and translational levels in L. lactis cells grown under aerobic condition. Presence of GSH and pyruvate caused more nisin yield than the heme-supplemented medium. Moreover, the expression of genes that encode stress-related enzymes were apparently upregulated in the presence of GSH and pyruvate. It can be concluded that GSH and pyruvate contribute to the defense system of L. lactis cells and so that higher biomass was obtained which in turn enhance nisin production. Antioxidant effect of GSH and pyruvate was known; however, their stimulating effect on nisin production was shown for the first time in this study.