Direct Atomic Absorption Spectrometric Determination of Selenium in Biological Fluids by a Graphite Filter Furnace Atomizer with Carbon Thread


Zacharia A., Gucer S., Arabadji M., Chebotarev A.

ANALYTICAL LETTERS, vol.51, no.15, pp.2390-2400, 2018 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 51 Issue: 15
  • Publication Date: 2018
  • Doi Number: 10.1080/00032719.2018.1424887
  • Journal Name: ANALYTICAL LETTERS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2390-2400
  • Bursa Uludag University Affiliated: Yes

Abstract

A rapid and simple method was developed for the determination of selenium in biological fluids by direct electrothermal atomic absorption spectroscopy. The operation parameters of a commercial longitude heating furnace combined with a graphite filter furnace, graphite thread (tube-in-tube atomization system), and a Pd-Mg chemical modifier were proposed, and the corresponding analytical characteristics for the determination of Se at concentrations greater than or equal to 0.0025mg/kg in blood, urine, saliva, and breast milk samples were established. Recovery values of Se with the proposed method varied within 93 to 107% for these matrices. Relative standard deviations for the analysis of certified material were also compatible; values of 5 and 7% were achieved for blood serum and urine, respectively. The relative standard deviation obtained by the proposed method did not exceed 8%, and the total time for Se determination was 5 to 7min. The novelty of this technique is that it offers an effective tool for removing the interferences from 10 to 20g/L organic and 1.0 to 1.5g/L mineral components in the materials and for significantly reducing the background absorption of light caused by their presence.