Accumulation profiles of PrPSc in hemal nodes of naturally and experimentally scrapie-infected sheep


Dassanayake R. P. , Truscott T. C. , ÖZYİĞİT M. Ö. , Zhuang D., Schneider D. A. , O'Rourke K. I.

BMC VETERINARY RESEARCH, vol.9, 2013 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 9
  • Publication Date: 2013
  • Doi Number: 10.1186/1746-6148-9-82
  • Title of Journal : BMC VETERINARY RESEARCH

Abstract

Background: In classical scrapie, the disease-associated abnormal isoform (PrPSc) of normal prion protein accumulates principally in the nervous system and lymphoid tissues of small ruminants. Lymph nodes traffic leukocytes via lymphatic and blood vasculatures but hemal nodes lack lymphatic vessels and thus traffic leukocytes only via the blood. Although PrPSc accumulation profiles are well-characterized in ovine lymphoid tissues, there is limited information on such profiles in hemal nodes. Therefore, the objective of this study was to compare the follicular accumulation of PrPSc within hemal nodes and lymph nodes by prion epitope mapping and western blot studies.