A comprehensive analysis of the effects of DGAT1 K232A polymorphism on milk production and fertility traits in Holstein Friesian and Jersey cows reared in Türkiye

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Ardicli S., Çobanoğlu Ö., Kul E., Abaci S. H., Gurcan E. K., Cankaya S.

ARCHIV FÜR TIERZUCHT, cilt.67, ss.455-467, 2024 (SCI-Expanded, Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 67
  • Basım Tarihi: 2024
  • Doi Numarası: 10.5194/aab-67-455-2024
  • Dergi Adı: ARCHIV FÜR TIERZUCHT
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts, Food Science & Technology Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Sayfa Sayıları: ss.455-467
  • Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

Research on the diacylglycerol acyltransferase 1 (DGAT1) K232A marker in cattle shows inconsistent

results across regions, largely due to small sample sizes, limited genetic variation, and data restricted to

few lactations, which complicates establishing a reliable genotype–phenotype correlation. This research aimed

to determine the effect of the K232A polymorphism of the bovine DGAT1 gene on milk production and quality

traits in dairy cattle. We used 1104 cattle, including 828 Holstein Friesian and 276 Jersey cows. The analysis

utilized extensive data from six lactations of cows raised on four commercial dairy farms. We genotyped the

population using the polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) technique

and Sanger sequencing for verification. We then evaluated the 305 d and test-day milk yields as well as

fat and protein yields and percentages. The number of inseminations per conception and calving ease were also

assessed as reproduction indices. Genotype–phenotype associations were quantified using linear mixed models.

The AA genotype was absent in Jersey cows, and the heterozygous genotype was predominant in both breeds.

The K232A marker was significantly associated with test-day milk yield, fat, and protein content in Jersey cows.

Further, it substantially affected the fat percentage of milk in Holstein Friesian cows (p<0:001). We found that

the KK genotype is highly desirable for milk quality and especially fat content. This comprehensive assessment

demonstrated that the KK genotype of the DGAT1 K232A polymorphism significantly influenced fat and protein

contents in dairy cattle.