Akademik Veri Yönetim Sistemi
International Journal of Science, Technology and Design, cilt.3, sa.1, ss.99-112, 2022 (Hakemli Dergi)
Microplastics have become a problem of the modern era with
the increasing use of plastics. However, the effects of
microplastics on living beings are not known exactly. As
stated in the booklet "Microplastics in Drinking Water"
published by the World Health Organization (WHO) in 2019,
there is no standard method for the detection of microplastics.
Although there are various analysis methods for the
determination of microplastics in the literature, some
deficiencies are observed. In this study, it is aimed to analyze
the microplastics in aquatic environment without using
organic solvents, especially in accordance with green
chemistry. In this context, Size Exclusion Chromatography
was used and the performance of four different Ultrahydrogel columns suitable for aqueous mobile phases was
tested. Polyethylene glycol (PEG) standards with different
molecular masses were used during this performance test. As
a result of the study, the theoretical plate numbers of Ultrahydrogel 250 Å, Ultra-hydrogel 500 Å, and Ultra-hydrogel
1000 Å columns were calculated as 256, 713, and 342,
respectively, and the regression coefficients (R2
) were
calculated as >0.97. The theoretical plate number of Ultrahydrogel 2000 Å column was not calculated because of lower
regression coefficient.