In vitro genotoxicity evaluation of acetamiprid in CaCo-2 cells using the micronucleus, comet and gamma H2AX foci assays


ÇAVAŞ T. , ÇİNKILIÇ N. , VATAN Ö. , YILMAZ D., Coskun M.

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, vol.104, no.3, pp.212-217, 2012 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 104 Issue: 3
  • Publication Date: 2012
  • Doi Number: 10.1016/j.pestbp.2012.08.004
  • Title of Journal : PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
  • Page Numbers: pp.212-217
  • Keywords: Acetamiprid, Genotoxic, CaCo-2, Comet, Micronucleus, gamma H2AX, DNA-DAMAGE, NEONICOTINOID INSECTICIDES, HUMAN-LYMPHOCYTES, CYTOTOXICITY, INDUCTION, PESTICIDES, TOXICOLOGY, RELEVANCE, APOPTOSIS, TOXICITY

Abstract

Acetamiprid is a member of the neonicotinoid group of insecticides commonly used against wide range of insect pests. In the present study, in vitro cytotoxicity and genotoxicity of technical grade acetamiprid was evaluated on the human intestinal CaCo-2 cells. Cytotoxicity was evaluated using the clonogenic survival and the results indicated that acetamiprid was cytotoxic on CaCo-2 cells. The cells were than treated with acetamiprid concentrations exhibit greater than 75% clonogenic survival for 24 h, to assess genotoxicity using the micronucleus, comet and gamma H2AX foci formation assays. Our results indicate that, under the experimental conditions used, acetamiprid has cytotoxic and genotoxic potential on human intestinal cells. (c) 2012 Elsevier Inc. All rights reserved.