The carotid canal must be taken into account during the surgical procedures since it contains the petrous segment of internal carotid artery. For this reason, we aimed to reveal the morphometric relationship of the carotid canal with related structures. The study was carried out with 11 parameters determined on a total of 52 sex unknown temporal bones belonging to the collection of Bursa Uludag University Faculty of Medicine, Anatomy Department. Measurements were taken by using mechanical sliding calipers and the findings were evaluated using the SPSS 22.0 program. Descriptive statistics of all variables were performed and correlation between the carotid canal and related structures were evaluated with the Spearman correlation analysis. Also, the correlation coefficient values were given. We developed the regression formulas to estimate the location of the carotid canal. There was a relationship between posterior and anterior point of external opening of carotid canal and mastoid process. The distance between the mastoid process-suprameatal spine and mastoid process-posterior root of zygomatic arch is highly correlated with each other.