Apoptosis-inducing effect of a palladium(II) saccharinate complex of terpyridine on human breast cancer cells in vitro and in vivo

ARI F., Cevatemre B., Armutak E. I. I., Aztopal N., YILMAZ V. T., Ulukaya E.

BIOORGANIC & MEDICINAL CHEMISTRY, cilt.22, sa.17, ss.4948-4954, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 22 Sayı: 17
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1016/j.bmc.2014.06.039
  • Dergi Adı: BIOORGANIC & MEDICINAL CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.4948-4954
  • Anahtar Kelimeler: Palladium(II) complex, Breast cancer, Apoptosis, Cytotoxicity, PD(II) COMPLEXES, DNA-BINDING, DEATH, CYTOTOXICITY, CASPASE-3, CLEAVAGE, CU(II), PT(II), ASSAY
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

The anti-growth effect of a palladium(II) complex-[PdCl(terpy)](sac)center dot 2H(2)O] (sac = saccharinate, and terpy = 2,2':6',2 ''-terpyridine)-was tested against human breast cancer cell lines, MCF-7 and MDA-MB-231. Anti-growth effect was assayed by the MTT and ATP viability assays in vitro and then confirmed on Balb/c mice in vivo. The mode of cell death was determined by both histological and biochemical methods. The Pd(II) complex had anti-growth effect on a dose dependent manner in vitro and in vivo. The cells died by apoptosis as evidenced by the pyknotic nucleus, cleavage of poly-(ADP-ribose) polymerase (PARP) and induction of active caspase-3. These results suggest that the palladium(II) saccharinate complex of terpyridine represents a potentially active novel complex for the breast cancer treatment, thus warrants further studies. (C) 2014 Elsevier Ltd. All rights reserved.