Inhibitory Effects of <i>Olea europaea</i> Leaf Extract on Mesenchymal Transition Mechanism in Glioblastoma Cells.


Mutlu M., TUNCA B. , Ak Aksoy S. , Tekin C., EGELİ Ü. , ÇEÇENER G.

Nutrition and cancer, vol.73, pp.713-720, 2021 (Journal Indexed in SCI Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 73
  • Publication Date: 2021
  • Doi Number: 10.1080/01635581.2020.1765260
  • Title of Journal : Nutrition and cancer
  • Page Numbers: pp.713-720

Abstract

Background: Glioblastoma (GB) is the most aggressive form of brain tumor. Despite the current treatment methods, the survival rate of patients is very low. Therefore, there is a need to develop new therapeutic agents. The migration and invasion capacity of GB cells is related to mesenchymal transition (MT) mechanism. Materials and Methods: The effect of OLE on MT was determined by analysis of the Twist, Snail, Zeb1, N-cadherin and E-cadherin genes in the EMT mechanism. The effect of OLE on cell migration was determined by wound healing test. Results: 2 mg/ml OLE reduced Twist, Snail, Zeb1 and N-cadherin expression and the combination of OLE + TMZ (2 mg/ml OLE + 350 mM TMZ) increased E-cadherin and reduced Twist, Zeb1 and N-cadherin. In addition, co-treatment with OLE increased TMZ-induced anti-invasion properties thought suppressing transcription factors of MT mechanism. Conclusion: OLE can enhance the anti-MT activities of TMZ against GB and provide strong evidence that combined treatment with OLE and TMZ has the potential to be an effective alternative approach in GB therapy.