Seroepidemiology and molecular investigation of pestiviruses among sheep and goats in Northwest Anatolia


Alpay G., Oner E. B., YEŞİLBAĞ K.

TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, cilt.42, sa.3, ss.205-210, 2018 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 42 Sayı: 3
  • Basım Tarihi: 2018
  • Doi Numarası: 10.3906/vet-1711-83
  • Dergi Adı: TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.205-210
  • Anahtar Kelimeler: Pestivirus, small ruminant, prevalence, RT-PCR, Ag-ELISA, virus neutralization, BOVINE VIRAL DIARRHEA, BORDER DISEASE VIRUS, RT-PCR, INFECTIONS, PIGS, SEROPREVALENCE, TRANSMISSION, ABORTION, EASTERN, CATTLE
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

Pestiviruses, which are distributed worldwide, may cause losses in sheep and goat populations. The current seroprevalence and circulation of pestiviruses in small ruminants of the South Marmara Region of Turkey were investigated. A total of 607 blood samples were collected from 22 different flocks in 4 different provinces, and 24 necropsy materials were investigated. Pestivirus seroprevalence in sheep and goats was determined by virus neutralization test against the NADL strain of bovine viral diarrhea virus. The mean antibody titer was 3.5, while the overall seropositivity rate was 32.78% (199/607). Seronegativity was markedly more prevalent in goats. The mean detected antibody titer was 6.8 in sheep and 1.1 in goats. All blood samples were negative as assessed by Ag-ELISA and RTPCR. Fourteen out of 24 fetal samples were detected as antigen-positive by Ag-ELISA. The amplification was observed using pan-pesti primers in four tissue samples. All four samples were identified as border disease virus by discriminative RT-PCR. In conclusion, the moderate seroprevalence but low antibody titers detected in this study indicate that small ruminants in the South Marmara Region may be at risk of extensive new circulations of pestivirus infections.