Establishment of a Canine Mammary Gland Tumor Cell Line Abstract


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Ardıçlı S. , Şamlı H. , Mecitoğlu G. , Vatansever B., Mutlu A. M.

7. Uluslararası Tıp ve Sağlık Bilimleri Araştırmaları Kongresi’nde (UTSAK) , Ankara, Turkey, 28 - 29 August 2021, vol.7, no.11, pp.97-98

  • Publication Type: Conference Paper / Summary Text
  • Volume: 7
  • City: Ankara
  • Country: Turkey
  • Page Numbers: pp.97-98

Abstract

Canine mammary gland tumors constitute 50–70% of all tumors in non-productive female dogs (6–14 years of age). The heterogenic nature of canine tumor cases causes unfavorable prognoses such as acquired resistance to therapy, recurrence, and metastasis. Insufficient variety of canine cell lines commercially available or difficulties in obtaining the existing cell lines developed from various studies results in a limited number of cytotoxicity and related molecular studies integrated with clinical practice. Hence, the doses of many drugs or supportive treatments used in canine tumor cases are adjusted based on studies in humans. In this study, a cell line was established from the mammary gland tumor of a 9 years old, female Cocker Spaniel dog. The tumoral material was obtained from the abdominal mammary gland by regional mastectomy. Histopathological examination revealed a diagnosis of canine benign mammary mixed tumor. Following mechanical dissociation and PBS washing, a culture process of tumor cells was performed, including the passaging, freezing, and thawing stages. After several passages, the morphological characteristics of the cells at the logarithmic growth phase were observed under a phase-contrast microscope. The average passage time, which was 5-6 days in the first three passages, decreased to 2-3 days after the third passage. Microscopic examination of tumor cells revealed an adherent, stellated, and spindle-shaped structure. No difference was observed in the viability and morphology of the cells thawed even after the long-period freezing (~18 months). This study represents the preliminary results of the project concerning the establishment and characterization of tumor cell lines to provide effective in vitro applications in both the diagnosis and treatment of canine mammary gland tumors. More comprehensive studies on cytotoxicity and genetic characterization of the obtained cell lines are now in progress.