Determination of Antiproliferative and Necroptotic Effects of Chalcone Compounds on Prostate Cancer Cells Kalkon Bileşiklerinin Prostat Kanseri Hücrelerinde Antiproliferatif ve Nekroptotik Etkilerinin Belirlenmesi
Journal of the Institute of Science and Technology, cilt.13, sa.3, ss.1552-1561, 2023 (Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 13 Sayı: 3
- Basım Tarihi: 2023
- Doi Numarası: 10.21597/jist.1298265
- Dergi Adı: Journal of the Institute of Science and Technology
- Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.1552-1561
- Anahtar Kelimeler: Anticancer, Antiproliferative, Chalcones, Necroptosis, Prostate cancer
- Bursa Uludağ Üniversitesi Adresli: Evet
Özet
Cancer is a multistage fatal disease that occurs with genetic and environmental conditions and uncontrolled division and proliferation of cells. Prostate cancer is one of the malignancies that affects men and contributes significantly to the increasing mortality rates in men globally. Due to a long disease history, genetic-phenotypic diversity, and uncertainty in the clinical progression of patients in prostate cancer, the necessity of developing new approaches arises. Chalcones are pharmacologically active compounds found in plants. It has been observed that natural or synthetic chalcone derivatives have anti-cancer activity in cancer cells. In the current study, the anti-cancer and anti-proliferative effects of two synthesized and characterized chalcone derivatives (Compound 1 and Compound 2) were investigated in human prostate cancer cell lines (LNCaP and PC-3). The anti-proliferative effect of the compounds on cell viability was assessed by SRB viability assay after 48 hours of treatment. Fluorescent staining (Hoechst 33342+Annexin-V+Propidium iodide) method was used to determine the cell death mode responsible for the cytotoxic effects of chalcone compounds, and RT-PCR analysis was performed to determine the changes in gene expressions. Anti-proliferative effects of the compounds were detected in LNCaP and PC-3 cells in a dose-and time-dependent manner. It was determined by triple fluorescent staining that the compounds induced secondary apoptosis in LNCaP and PC-3 cells. Significant increases in expression levels of cell death pathway genes BCL-2, MLKL, FAS and PARP were determined. In the light of the results obtained, it was concluded that Compound 1 and Compound 2 showed anti-proliferative effect and induced necroptosis in prostate cancer.