Effects of Systemic Probiotic Administration on Lens in Diabetic Rats


Akova B. , Kıvanç S. A.

ASCRS 2022, Washington, United States Of America, 22 - 26 April 2022, vol.1, no.1, pp.1

  • Publication Type: Conference Paper / Summary Text
  • Volume: 1
  • City: Washington
  • Country: United States Of America
  • Page Numbers: pp.1

Abstract

Purpose
Diabetes mellitus has long been known to cause cataract formation. In this study our aim was to investigate the effects of systemic probiotics at gene expression level on the crystalline lenses of the diabetic rats.

Methods
Twenty-four Sprague Dawley rats were included in the study. The rats were divided into 4 groups as diabetic rats, diabetic rats with probiotic intake, rats with probiotic intake and control group. The diabetes mellitus was induced by streptozocin 20mg/kg. The systemic probiotic used in the study was Lactobacillus rhamnossus312. After 2 months the lenses of the rats were removed. Total RNA isolation from the lens material was performed with total RNA purelinkRNA minikit. The amount of RNA was detected by Qubit fluorometer. The genes used for the study were VEGF, IL-1beta, Aldolaz, NF-KB.

Results
Cataract formation was evaluated under light microscopy all animals had cataract formation in diabetic rats group. Cataract formation rate was lower in diabetic rats with probiotic intake. The gene expression values of VEGF, NF-KB, Aldolaz on crystalline lenses were significantly different among groups.(p=0.029, p<0.001, 0.003 respectively, One Way Anova)

Conclusion
Systemic probiotic administration may reduce cataract formation and may have protective effects on lens in diabetic rats.