Acid proteinase enzyme activity in Candida albicans strains: a comparison of spectrophotometry and plate methods

AKÇAĞLAR S., ENER B., Tore O.

TURKISH JOURNAL OF BIOLOGY, vol.35, no.5, pp.559-567, 2011 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 35 Issue: 5
  • Publication Date: 2011
  • Doi Number: 10.3906/biy-1002-39
  • Journal Name: TURKISH JOURNAL OF BIOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.559-567
  • Keywords: Candida albicans, secreted aspartyl proteinase (SAP) activity, virulence, BLOOD-STREAM INFECTIONS, PHOSPHOLIPASE-ACTIVITY, VIRULENCE FACTORS, UNITED-STATES, VAGINITIS, SUSCEPTIBILITY, PATHOGENICITY, FREQUENCY
  • Bursa Uludag University Affiliated: Yes

Abstract

In recent years, the incidence of fungal infections has been rising all over the world. Although the amount of research in the field of pathogenic fungi has also increased, there is still a need for studies on fungal virulence. In this investigation, we focused on testing bloodstream-identified Candida albicans in experimental in vitro virulence assays. The proteinase enzyme activities of 30 C. albicans strains isolated from the bloodstream and sterile body fluids were investigated (15 colonizations, 15 infections). The spectrophotometric and plate methods were used to determine proteinase activity. The correlations between the 2 methods were compared. This study indicates that, in terms of proteinase activity, there were no statistically significant differences between strains obtained from infection and colonization sites by either method (P > 0.05).