A Study on the Anticarcinogenic Effects of Calcium Fructoborate

Tepedelen B., Korkmaz M., Tatlisumak E., Uluer E. T., Olmez E., Degerli I., ...Daha Fazla

BIOLOGICAL TRACE ELEMENT RESEARCH, cilt.178, sa.2, ss.210-217, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 178 Sayı: 2
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1007/s12011-016-0918-6
  • Dergi Adı: BIOLOGICAL TRACE ELEMENT RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.210-217
  • Anahtar Kelimeler: MDA-MB-231, Boron, Calcium fructoborate, DNA damage, Apoptosis, VEGF, INHIBITS HUMAN PROSTATE, BREAST-CANCER, DIETARY BORON, BORIC-ACID, PHENYLBORONIC ACID, GROWTH, SUPPLEMENTATION, MECHANISMS, APOPTOSIS, MIGRATION
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

Evidences about the preventive and therapeutic effects of boron compounds on cancer have been increasing in the last years. Although calcium fructoborate (CaFB) is used as a nutritional supplement, data about its preventive and therapeutic effects on neoplastic transformations are limited. In the present study, the various concentrations of CaFB were applied to the MDA-MB-231 metastatic breast cancer cell line. First, we examined the cytotoxic effect and IC50 value of CaFB by MTT assay. For the evaluation of the DNA damage, apoptosis and metastatic potential, expression levels of ATM, pATM, PARP, p53, p-p53, caspase-3, caspase-9, and VEGF were investigated by using immunoblotting and immunohistochemical methods. Cell viability was significantly reduced at 50 mu M CaFB treatment. pATM, p-p53, and caspase-9 levels increased significantly in all groups; furthermore, there was approximately 12.5-, 2.4-, and 10.7-fold increase, respectively, for 100 mu M CaFB treatment. ATM and p53 levels did not change with CaFB treatment, but PARP levels significantly 2.5-fold decreased. While VEGF immunoreactivity decreased in all groups, significant increase in caspase-3 immunoreactivity was observed only in the group treated with 50 mu M CaFB ( p < 0,001). Our results imply that CaFB may have therapeutic potential as well as preventive benefits in cancer.