An Investigation on Gene Regions Related to Some Economic Traits in Holstein and Brown Swiss Cattle Breeds by Using PCR-RFLP Technique

Sahin S., ÖNER Y., ELMACI C.

JOURNAL OF AGRICULTURAL SCIENCES-TARIM BILIMLERI DERGISI, vol.19, no.3, pp.235-244, 2013 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 19 Issue: 3
  • Publication Date: 2013
  • Doi Number: 10.1501/tarimbil_0000001248
  • Journal Name: JOURNAL OF AGRICULTURAL SCIENCES-TARIM BILIMLERI DERGISI
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.235-244
  • Keywords: Cattle, Polymorphism, CAPN1, ER alpha, PRL, MSTN, TISSUE-SPECIFIC EXPRESSION, BOVINE PROLACTIN GENE, GROWTH-HORMONE, ESTROGEN-RECEPTORS, MEAT TENDERNESS, KAPPA-CASEIN, ALPHA GENE, ER-ALPHA, POLYMORPHISM, ASSOCIATION
  • Bursa Uludag University Affiliated: Yes

Abstract

In this study, the polymorphisms in calpain (CAPN1), estrogen receptor alpha (ER alpha), prolactin (PRL), and myostatin (MSTN) genes thought to be related to some economical traits were investigated in 136 heads from Holstein and Brown Swiss cattle breeds reared in Karacabey town of Bursa by using PCR-RFLP method. While MSTN locus was found as monomorphic, 2 alleles of each CAPN1, ER alpha and PRL genes were determined in both breeds. While 3 genotypes (CC, CT, and TT) were determined at CAPN1 locus of both breeds, 2 genotypes (AG and GG) and 3 genotypes (AA, AG, and GG) were determined at PRL locus of Holstein and Brown Swiss breeds, respectively. Two genotypes (AG and GG) were detected at ER alpha locus in both breeds. Both two populations were found at Hardy-Weinberg equilibrium except for PRL locus in Brown Swiss population.