Lack of association of genetic polymorphisms of angiotensin-converting enzyme gene i/d and glutathione-s-transferase enzyme t1 and m1 with retinopathy of prematures

Yildiz M., Karkucak M., Yakut T., Gorukmez O., Ozmen A.

Genetics and Molecular Research, vol.9, no.4, pp.2131-2139, 2010 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 9 Issue: 4
  • Publication Date: 2010
  • Doi Number: 10.4238/vol9-4gmr887
  • Journal Name: Genetics and Molecular Research
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2131-2139
  • Keywords: Polymorphism, ACE gene, GSTT1, GSTM1, Retinopathy of prematurity, NORRIE-DISEASE GENE, ENDOTHELIAL GROWTH-FACTOR, BIRTH-WEIGHT INFANTS, INSERTION/DELETION POLYMORPHISM, MISSENSE MUTATIONS, NULL GENOTYPES, SLEEP-APNEA, RISK, PROGRESSION, FIBRINOLYSIS
  • Bursa Uludag University Affiliated: Yes

Abstract

One of the most frequently observed causes of blindness in infancy is the pathogenesis known as retinopathy of prematurity (ROP). Angiotensin-converting enzyme (ACE) is a vital enzyme in the renin-angiotensin-aldosterone system; it is involved in the development of cardiovascular system diseases linked to I/D polymorphism of the ACE gene. Glutathione-S-transferase enzyme (GST) is one of the most important regulating components of the antioxidant system; there are indications that certain polymorphisms of GST genes (GSTT1, GSTM1), especially the null genotypes, increase the tendency for oxidative stress diseases. We investigated a possible correlation between ACE gene I/D and GSTT1 and GSTM1 gene polymorphisms in 56 prematures suffering from ROP and a control group composed of 48 prematures without ROP in a hospital in Turkey. PCR was used to detect the ACE I/D, GSTT1 and GSTM1 gene polymorphisms. Genotype was determined based on bands formed on agarose gel electrophoresis. We found no significant differences in genotype frequency of the ACE I/D, GSTT1 and GSTM1 genes between normal subjects and patients with ROP. Our results do not support an association of ACE I/D, GSTT1 and GSTM1 gene polymorphisms with risk for ROP. © FUNPEC-RP.