Tarantula cubensis extract (Theranekron (R)) inhibits inflammation in carrageenan-induced acute paw edema in rats


Creative Commons License

Ozfirat E. C., Topal A., Akkoc A., Satar N. Y. G., Ipek V., Pamukcular O., ...Daha Fazla

ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA, cilt.74, sa.1, ss.56-64, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 74 Sayı: 1
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1590/1678-4162-12550
  • Dergi Adı: ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, Food Science & Technology Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Sayfa Sayıları: ss.56-64
  • Anahtar Kelimeler: inflammation, paw edema, rat, Tarantula cubensis, EFFICACY, CATTLE, TRIAL, VENOM
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

The aim of this study was to investigate the anti-inflammatory effect of alcoholic extract of Tarantula cubensis alcoholic extract (TCAE) in experimentally induced inflammation in rats. Fifty-four adult Sprague-Dawley male rats were randomly divided into nine groups. Paw edema was induced by 0.2mL subplantar (s.p.) injection of 1% carrageenan (CAR) into the right hind paw. Rats were treated with the nonsteroidal anti-inflammatory drug (NSAID) indomethacin (INDO) (10mg/kg, p.o.) or TCAE at different doses (1, 10 or 100 mu g/kg) injected s.c. for systemic or s.p. for local anti-inflammatory effect. Saline was used as control. Changes in paw thickness, volume, and weight were calculated as percentages. Formalin-fixed paws were used for histopathological examination. We detected that TCAE applied s.c. at 10 mu g/kg and 100 mu g/kg doses resulted in thinner paw thickness, lower paw volume, and lower paw weights four hours after the induction of inflammation when compared with the INDO group (p<0.05). The paw edema inhibitory effect of TCAE applied at a dose of 10 mu g/kg, s.c. was 68% when compared with the INDO which had an inhibitory effect of 56%. These results were verified with similar histopathological findings. The anti-inflammatory feature of 10 mu g/kg of TCAE given systematically was similar to the effects of INDO. Our results suggest that TCAE has anti-inflammatory effects by reducing edema and decreasing inflammatory reaction. These results may be attributed to the inhibition of the production of proinflammatory mediators. Thus, TCAE may be considered as a potential anti-inflammatory agent for treating acute inflammatory conditions.