TURKISH JOURNAL OF AGRICULTURE: FOOD SCIENCE AND TECHNOLOGY, cilt.10, sa.6, ss.992-996, 2022 (Hakemli Dergi)
Changing climatic conditions and growing population size lead take precautions against potential
risks. Researchers who study in the agricultural field, including animal production, focus on finding
out variants and breeds resistant to diseases and environmental stress. Detecting gene regions that
affect resistance to diseases and environmental stress might increase the performance of the selection
in favor of these traits. Due to these reasons in this study, five Turkish native cattle breeds were
investigated for SNPs belonging to TLR2, NOD2, and IFNγ (using EcoRV, BsaHI ve HphI enzymes,
respectively) reported to be associated with paratuberculosis previously. In total, 169 animals were
analyzed from Native Southern Yellow (NSY= 29), East Anatolian Red (EAR=35), Anatolian Grey
(AG=36), South Anatolian Red (SAR=34), and Native Black (NB=35). The genotype and allele
frequencies were calculated with the PopGene32 program, and Khi square test was performed to
determine whether the populations were in Hardy-Weinberg equilibrium (HWE). While at TLR2-
EcoRV and NOD2- BsaHI loci, two alleles (A and C, C and T, respectively), IFNγ- HphI locus was
found to be monomorphic for the G allele. For TLR2- EcoRV locus, the C allele was found as
predominant except NB breed, and the C allele was predominant for all breeds at NOD2- BsaHI locus.
However, in all breeds, NOD2- BsaHI locus was at HWE, for TLR2- EcoRV NSY and SAR breeds
showed derivation from HWE