Akademik Veri Yönetim Sistemi
Swiss Medical Weekly, cilt.154, sa.9, ss.23-24, 2024 (SCI-Expanded)
Aim: The importance
of immunomodulation in bone healing is widely recognized, and the field of
osteoimmunology is rapidly growing in significance. Large bone defects often
need additional material to fill voids and support bone formation. In this
study, we investigate the immunomodulatory properties of novel bone-forming
materials using high-throughput techniques.
Methods: The response
of PBMCs to hydrogels based on tyramine-modified hyaluronic acid gel (THA),
agarose, and fibrin sealant, as well as beta-tricalcium phosphate and hyaluronic
acid particles, was examined in monolayer and transwell cultures. Our
experiments included measuring cell proliferation using thymidine assay, cell
viability with propidium iodide in flow cytometry, and targeted proteomics
using Proximity Extension Assay.
Results: The data
showed a slight increase in cell proliferation when exposed to THA gels,
agarose, or fibrin sealant. In the targeted proteomics data, fibrin sealant and
bone particle combinations led to upregulated proteins associated with bone
remodeling and low inflammatory response. Conditions related to agarose and
bone particles did not induce significant alterations in our biomarker panels. We
identified the matrisome, plasmacytoma, apoptosis, and immune response-related
pathways for the fibrin sealant as well as its combination with filtrated bone
particles.
Conclusions: PBMC
response to fibrin sealant and its combination with filtered bone particles
point out reduced bone resorption and potentially increased bone formation.