Towards deciphering how inflammatory response to biomaterials modulates bone formation


Ardıçlı S., Wawrocki S., Bektas E. I., Babayev H., Ardıçlı Ö., Pat Y., ...More

Swiss Medical Weekly, vol.154, no.9, pp.23-24, 2024 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 154 Issue: 9
  • Publication Date: 2024
  • Doi Number: 10.57187/s.4176
  • Journal Name: Swiss Medical Weekly
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, MEDLINE, Directory of Open Access Journals
  • Page Numbers: pp.23-24
  • Bursa Uludag University Affiliated: Yes

Abstract

Aim: The importance of immunomodulation in bone healing is widely recognized, and the field of osteoimmunology is rapidly growing in significance. Large bone defects often need additional material to fill voids and support bone formation. In this study, we investigate the immunomodulatory properties of novel bone-forming materials using high-throughput techniques.

 

Methods: The response of PBMCs to hydrogels based on tyramine-modified hyaluronic acid gel (THA), agarose, and fibrin sealant, as well as beta-tricalcium phosphate and hyaluronic acid particles, was examined in monolayer and transwell cultures. Our experiments included measuring cell proliferation using thymidine assay, cell viability with propidium iodide in flow cytometry, and targeted proteomics using Proximity Extension Assay.

 

Results: The data showed a slight increase in cell proliferation when exposed to THA gels, agarose, or fibrin sealant. In the targeted proteomics data, fibrin sealant and bone particle combinations led to upregulated proteins associated with bone remodeling and low inflammatory response. Conditions related to agarose and bone particles did not induce significant alterations in our biomarker panels. We identified the matrisome, plasmacytoma, apoptosis, and immune response-related pathways for the fibrin sealant as well as its combination with filtrated bone particles.

 

Conclusions: PBMC response to fibrin sealant and its combination with filtered bone particles point out reduced bone resorption and potentially increased bone formation.