This study aimed to determine the presence of Campylobacter coli in water lines of commercial broiler houses. The samples were taken in the period of three sequential flocks in two houses. There was a distance of approximately 25 km between the houses and drinking water was supplied by groundwater in one house while the municipal water system with polyvinyl chloride plastic pipe was the source of drinking water in the other. C. coli was isolated and identified in drinking water and nipple swab samples, and fresh fecal dropping samples in both houses. The results of this study suggest that C. coil could be found in water-line of commercial broiler houses and drinking water might be a source for flock's colonization by C. coli.