Akademik Veri Yönetim Sistemi
2. International Cancer and Ion Channel Congress , İzmir, Türkiye, 22 - 24 Eylül 2019, ss.65
IS THERE ANY CO-CHANGES BETWEEN VOLTAGE-GATED Na+ CHANNEL SCN5A AND CTNNB1 GENES IN COLORECTAL CANCER? Yunus TOSOI, Handan TUNCELI ICerrahpasa Medical Faculty, Istanbul University- Cerrahpasa, Turkey Objectives: New evidence suggests that ion channels play important roles in cell proliferation, migration, apoptosis and differentiation. NaV1.5 encoded by the SCN5a gene. Also, mutations or polymorphisms in CTNNB1 (gene encoding b-catenin; mostly mutations in exon 3) can lead to aberrant activation of Wnt/b-catenin signaling at the onset of various types of malignancies. The aim of this study was to assess relation between the SCN5A gene and CTNNB1alteration in the patients with colorectal cancer and compared their tumor and normal tissues. Materials and Methods: A total of 30 paraffin-embedded colorectal cancer and normal tissue specimens were used. Ten-micrometer-thick tissue sections were placed on a glass slide. DNA was extracted from the tissues with extraction buffer at 55°C over night. The tubes were boiled to inactivate the proteinase K. The SCN5A exon 18 and CTNNB1genotypes were determined by PCR amplification. PCR products of SCN5A exon 18 were digested with restriction enzyme BseRI. SSCP is used for CTNNB1 exon 3 to observe any difference between the 2 groups. The gel was stained with silver staining method. Results: In our study no significant differences were found in the CTNNB1 exon 3 and SCN5A exon 18, between the tumor group and normal groups, also any association with two sides. Conclusions: Adhesion involve continuous modulation of cell motility, ion channels play major roles. We need urgently much more work with large sample sizes are required for the association Na+ channels with adhesion molecules. Keywords: CTNNB1, SCN5A, Colorectal cancer, SSCP, RFLP