Real-time PCR culture and serology for the diagnosis of Mycoplasma gallisepticum in chicken breeder flocks


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Kahya Demirbilek S. , Temelli S. , Eyigor A., Carlı K. T.

VETERINARY MICROBIOLOGY, vol.144, pp.319-324, 2010 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 144
  • Publication Date: 2010
  • Doi Number: 10.1016/j.vetmic.2010.01.012
  • Title of Journal : VETERINARY MICROBIOLOGY
  • Page Numbers: pp.319-324
  • Keywords: Mycoplasma gallisepticum, Real-time PCR, Chicken, POLYMERASE-CHAIN-REACTION, AVIAN MYCOPLASMAS, SYNOVIAE, STRAIN, ASSAY

Abstract

This study aimed to compare a real-time PCR (rPCR) test with improved detection limit to serology and culture for the detection of Mycoplasma gallisepticum (MG) infection in chicken breeder flocks. Six hundred and forty-six blood and tracheal swab samples belonging to 31 grandparent chicken breeder flocks were tested by rPCR. The detection limit of rPCR was 0.9 pg mu l(-1), with pure MG S6 strain DNA and 100 colony forming units (CFUs) ml(-1), where both pure culture and tracheal swabs were artificially spiked with the same strain.