Maternal Gut Dysbiosis Exacerbates Offspring Susceptibility to Collagen-Induced Arthritis in Mice

Creative Commons License

Dündar F., Arslan G., Yumuşak E., Atılğan M., Ermiş D. Y., Akkoç A., ...More

Turkish Journal of Immunology, vol.14, no.2, pp.75-85, 2026 (ESCI, Scopus)

  • Publication Type: Article / Article
  • Volume: 14 Issue: 2
  • Publication Date: 2026
  • Doi Number: 10.36519/tji.2026.1183
  • Journal Name: Turkish Journal of Immunology
  • Journal Indexes: Emerging Sources Citation Index (ESCI), Scopus, EMBASE, Directory of Open Access Journals, Academic Search Ultimate (EBSCO)
  • Page Numbers: pp.75-85
  • Keywords: autoimmunity, collagen-induced arthritis, dysbiosis, fecal microbiota transplantation, Gut microbiota, rheumatoid arthritis
  • Open Archive Collection: AVESIS Open Access Collection
  • Bursa Uludag University Affiliated: Yes

Abstract

Objective: The gut microbiota plays a critical role in immune system development and the maintenance of immune tolerance. Dysbiosis, particularly during the maternal period, has been implicated in autoimmune diseases. This study investigated whether maternal antibiotic-induced dysbiosis exacerbates collagen-induced arthritis (CIA) in offspring and whether fecal microbiota transplantation (FMT) can reverse this effect. Materials and Methods: To establish a dysbiosis model, pregnant BALB/c mice were administered a combination of amoxicillin (40 mg/kg) and vancomycin (30 mg/kg) from the last week of pregnancy until the male pups used in the experiment (n=5 per group) reached 8 weeks of age (total treatment duration, 9 weeks). In the FMT group, oral FMT was administered after completion of antibiotic therapy. The bacterial profiles of the mice gut microbiota were analyzed by sequencing, and CIA was subsequently induced in all groups. Results: Maternal dysbiosis significantly reduced bacterial populations in the gut microbiota, whereas FMT restored these populations. Additionally, dysbiosis was associated with increased arthritis severity in the CIA model, whereas FMT appeared to reduce it. These results demonstrate that the maternal gut microbiota plays a critical role in the pathogenesis of autoimmune arthritis and that FMT may represent a potential therapeutic approach. Conclusion: Maternal dysbiosis in the gut microbiota and its treatment with FMT affected body weight in mice, particularly causing low birth weight. Dysbiosis increased disease severity in the CIA model, whereas FMT treatment reduced it.