Research Information System
FEMS MICROBIOLOGY LETTERS, vol.1, no.369, pp.1-6, 2022 (SCI-Expanded)
Slx5, a subunit of a SUMO-targeted ubiquitin ligase (STUbL) in yeast,
has been implicated in maintenance of genomic stability. SUMOylation is
an important post-translational modification involved in the regulation
of several important cellular processes and cellular response to various
environmental stressors. Oxidative stress occurs when production of
reactive oxygen species (ROS) exceeds the antioxidant defense capacity
of the cell. Elevated ROS levels cause oxidative damage to important
cellular macromolecules such as DNA, lipids, and proteins, which is
associated with several diseases. Herein, we investigated the role of
Slx5 in oxidative stress tolerance in Saccharomyces cerevisiae. We show that deletion of SLX5 increases survival of yeast cells in response to H2O2-induced
oxidative stress in a cell cycle independent manner. Accumulation of
intracellular ROS as well as DNA and lipid damages were reduced;
expressions of antioxidant defense mechanism-related genes were
increased in slx5Δ cells compared to wild type (WT) under oxidative stress. We also show that slx5Δ
cells have increased intracellular ROS levels and oxidative damage to
DNA and lipids compared to WT in the absence of oxidative stress. Thus,
our data together suggest that an adaptive stress induced by SLX5 deletion increases tolerance to oxidative stress in slx5∆ cells.