Expression Profile of Prostaglandin Enzymes in Cystic Endometrial Hyperplasia in Dogs: The Results of a Hypothesis in Clinical Trial Köpeklerde Kistik Endometriyal Hiperplazide Prostaglandin Enzimlerinin Ekspresyon Profili: Klinik Deneylerde Bir Hipotezin Sonuçları

Korlu Y., Yavaş Ö., Aktar A., Bozkurt B., ÖZYİĞİT M. Ö., Özalp G. R.

Kafkas Universitesi Veteriner Fakultesi Dergisi, cilt.29, sa.1, ss.21-31, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 29 Sayı: 1
  • Basım Tarihi: 2023
  • Doi Numarası: 10.9775/kvfd.2022.28403
  • Dergi Adı: Kafkas Universitesi Veteriner Fakultesi Dergisi
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, EMBASE, Veterinary Science Database, Directory of Open Access Journals, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.21-31
  • Anahtar Kelimeler: Canine, Cystic endometrial hyperplasia, Estrogen receptor, Prostaglandin, TOLL-LIKE RECEPTORS, PYOMETRA, CANINE, CYCLE, CYCLOOXYGENASE-2, ESTROGEN, BITCH
  • Bursa Uludağ Üniversitesi Adresli: Evet

Özet

The expressions of prostaglandin synthesis enzymes and estrogen, progesterone receptors in canine cystic endometrial hyperplasia (CEH) were reported in this manuscript. Uterine tissue samples were collected from bitches with CEH (n=5), CEH-P (Cystic endometrial hyperplasia-Pyometra) (n=5) and healthy-negative control group, CG (n=5). Immunohistochemistry was carried out for the estrogen (ER) and progesterone receptor (PR) detection. Shock-frozen samples were utilized in mRNA extraction and Real-Time PCR was performed. Gene expression of PTGS2/COX2, PTGES, PTGER4, PGFS, PTGFR and PGR were detected higher in the CEH group compared with CG. The PGFS and PTGFR (FP) mRNA expressions were significantly increased in CEH compared with other groups. Expression of progesterone receptor mRNA (PGR) was highest in CEH and statistically different from the CEH-P group (P<0.05). No PR immunostaining was observed. ER staining had been detected in endometrial glands, endometrial stoma and myometrium, however hyperplasic glands in propria mucosa had lower or no ER scores. Based on the results of this study, the high levels of prostaglandin enzymes and low ER scores in CEH could be a preliminary step for the next stages of severe differentiation of endometrium.